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Fluorescence Filters
Interference filters are essential optical components
for fluorescence measurements in life science and biophotonics. These
measurements require a pair of filters: the first filter (exciter filter )
for the transmission of the excitation beam to cause the absorption in the
sample and the second filter (emitter filter) for the fluorescent color to pass and
meanwhile block the residual excitation energy to get into the detector. Our high performance filter
sets are designed for a large variety of fluorophores and have a high
peak transmissions of >92% in the pass band and OD6 blocking in the
stop band.
Learn more about thin
film filters
Standard Fluorescence Filter Sets
|
Part # |
Fluorophore |
Exciter |
Emitter |
Center
Wavelength |
Typical
Bandwidth |
Center
Wavelength |
Typical
Bandwidth |
| FF-DAPI |
DAPI |
365 nm |
40 nm |
460 nm |
50 nm |
| FF-CFP |
CFP |
436 nm |
20 nm |
480 nm |
30 nm |
| FF-GFP |
GFP |
470 nm |
40 nm |
525 nm |
50 nm |
| FF-FITC |
FITC |
480 nm |
30 nm |
535 nm |
40 nm |
| FF-YFP |
YFP |
500 nm |
20 nm |
543 nm |
30 nm |
| FF-TRIT |
TRITC |
530 nm |
50 nm |
605 nm |
55 nm |
| FF-CY3 |
CY3
|
545 nm |
30 nm |
610 nm |
75 nm |
| FF-TEXR |
Texas RedR |
560 nm |
40 nm |
630 nm |
60 nm |
| FF-CY5 |
CY5
|
620 nm |
60 nm |
695 nm |
70 nm |
Exciter/Emitter Filter Specifications
| Substrate Material: |
Low fluorescent BK7 glass or equivalent |
| Substrate Size: |
Diameter: 10 or 15+0.0/-0.1 mm, Thickness: 3.3 ± 0.1 mm |
| Holder Size: |
Diameter: 25 +0.0/-0.1 mm, Thickness: 5.0 ± 0.1 mm |
| Passband
Transmission: |
>92% (except DAPI) |
| Reflection Band: |
N/A |
| Surface Quality: |
60/40 scratch and dig |
| Clear Aperture: |
>85% of central area |
| Blocking: |
OD6 outside of pass band
|
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